the Gingras Laboratory

The Lunenfeld-Tanenbaum Research Institute

 

Resources

Protocols

Affinity-purification coupled to mass spectrometry in human cells
Sources: Gingras et al., Mol Cell Proteomics, 2005;
Chen and Gingras, Methods, 2007;
Chen et al., J Biol Chem, 2008;
Goudreault et al, Mol Cell Proteomics, 2009
Mammalian TAP AP-MS
Mammalian FLAG AP-MS

Mammlian FLAG AP-MS Protocols
Source: Kean et al., Methods, In press.
Anti-FLAG AP-MS with Magnetic beads 2012.
Anti-FLAG AP-MS with Agarose beads 2012.
293 Flp-In FLAG magnetic AP - July 2013.
Affinity-purification coupled to mass spectrometry in S. cerevisiae
Sources:
Breitkreutz et al., Science, 2010

Yeast TAP AP-MS
Yeast HA or FLAG AP-MS
293 Flp-In FLAG magnetic chromatin optimized AP protocol
Developed by Jean-Philippe Lambert

293 Flp-In FLAG magnetic chromatin optimized AP - July 2013.

Flp-In BioID Streptavidin sepharose protocol
Implemented by Amber Couzens

Flp-In BioID Streptavidin sepharose - Jul 2013.

Flp-In BioID Streptavidin sepharose chromatin optizided
Developed by Jean-Philippe Lambert

Flp-In BioID Streptavidin sepharose chromatin optizided.

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